While the proportion of people infected with the Human Immunodeficiency Virus (HIV) dying of Acquired Immunodeficiency Syndrome (AIDS) has decreased significantly over the past four decades as antiretroviral therapy has become more common, the full structure of the HIV-1 genome remains unsolved. The 5′ leader of the RNA sequence is of particular interest to solve, as it is the most highly conserved region of HIV’s genome and plays a key role in the packaging and proliferation of the virus. NMR spectroscopy is our lab’s method of elucidating RNA complexes due to its ability to collect data on dynamic complexes in solution. Our group will be using a U1A reporter protein paramagnetically tagged by M8-DOTA-SPy to induce long-range pseudocontact shifts (PCS) in the NMR spectra of our RNA construct. The goal of this project is to elucidate the tertiary structure of the orientation of the TAR and polyA stems of the 5′ leader in relation to each other to build on the Brown et. al paper published in 2020. We will show binding through electrophoretic mobility shift assays (EMSAs) and show PCS of a TAR polyA construct.
Student support provided by the National Institute of General Medical Sciences, National Institutes of Health (NIGMS/NIH) under National Research Service Award T34 GM 136497, NIGMS Initiative for Maximizing Student Development Grant (2R25-GM55036), NIAID grant ##8 R01 AI150498, and the Howard Hughes Medical Institute.
Autism Spectrum Disorder (ASD) is, at once, one of the most common developmental disorders in the world and one of the most debilitating, however, its exact causes remain poorly understood. A preliminary genetic screen has identified the gene CaMKI as a molecular candidate involved in ASD. We hypothesize that mutations in CaMKI would lead to ASD-phenotypes such as sociability deficits and aberrant synaptic refinement. Using Drosophila as a model organism, we genetically ablated CaMKI and used a social assay to measure any resulting social impairment in adult flies, a hallmark of ASD in humans. Additionally, the role of CaMKI in synaptic refinement was tested at the larval neuromuscular junction (NMJ) as well as in the central nervous system. Furthermore, we live-imaged CaMKI-null embryos expressing the genetically encoded calcium indicator GCAMP to examine the activity pattern of the nociceptive class-IV (C4da) sensory cells. Our results suggest that this activity pattern is necessary for the proper development of neuronal networks, so any irregularities provide direct evidence of impeded maturation via synaptic refinement. This study explores the relationship between CaMKI and ASD which casts light on any connection between the disorder and developmental synaptic refinement, as a whole.
This work was funded, in part, through an Undergraduate Research Award from the UMBC Division of Undergraduate Academic Affairs.
The COVID-19 pandemic has caused various parts of our society to move online and in turn served to further amplify the digital divide in that lacking reliable broadband connectivity can have a larger impact now more than ever before. As so many facets of the world have continued to shift into the digital world in recent years, members of our society without access to high-quality internet connectivity are prevented from being able to fully participate in this new world. The arrival of COVID-19 magnified this problem since so many services and social activities have moved online. We explored the impact of not having reliable high-speed home Internet connectivity on families in Baltimore City, with a specific focus on the impact on educational, employment, social activities, and health. We conducted pilot interviews with community participants, stakeholders working to alleviate this issue by setting up community Internet connectivity resources, and informal educators working in this space. To date, we have found that participants value connectivity and also see the importance of developing reputable, safe, and inclusive resources to ease a move online for families who are just receiving broadband Internet.
This work was funded, in part, by the National Science Foundation.
This work was funded, in part, by the Alex Brown Center for Entrepreneurship URA.
URAs provide up to $1,500 to undergraduate students to support their research or creative work with a UMBC faculty mentor on an original project. UMBC students of all years and disciplines are invited to apply, as long as they will remain enrolled at UMBC long enough to complete the proposed work.
Apply here:
Applications require a research proposal and mentor statement of support.
Questions: aprilh@umbc.edu
URAs provide up to $1,500 to undergraduate students to support their research or creative work with a UMBC faculty mentor on an original project. UMBC students of all years and disciplines are invited to apply, as long as they will remain enrolled at UMBC long enough to complete the proposed work.
Start your process here:
There are many changes happening to student research as a result of the Covid-19 pandemic and campus closure. The URA selection committee has some advice to encourage you to apply for a URA and start to plan your research, even if there are some unknowns for the summer and fall semesters.
Applications require a research proposal and mentor statement of support.
Questions: aprilh@umbc.edu
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