Name: Linda Wiratan

Internship, Co-op or Research Site: UMBC Department of Chemistry & Biochemistry

Position Title: Research Assistant

Major(s)/Minor(s): B.S. Biochemistry & Molecular Biology; Honors College

Expected Graduation Year: May 2019

Briefly describe your internship, co-op,  or research opportunity, including your day-to-day tasks, responsibilities, and assignments.

I am a member of Dr. Ptaszek's organic chemistry research group (at the UMBC Department of Chemistry & Biochemistry), whose focus is on the development of hydroporphyrins (chlorins and bacteriochlorins) for a wide variety of optical and photochemical applications, particularly photodynamic therapy (PDT). PDT is a prospective cancer therapy that involves the use of a photosensitizer (light-activated probe) to localize within and kill cancer cells through the formation of toxic species. Hydroporphyrins are relatives of the familiar compounds heme and chlorophyll, and their properties can be tuned to give them potential to become PDT probes.

My daily responsibilities are carrying out experiments pertaining to my specific research project, which examines the dependence of specific hydroporphyrin properties on solvent polarity. I typically measure absorbance and emission scans for a variety of hydroporphyrins in different solvents, analyzing the data using Excel and Kaleidagraph. I look for correlations between solvent polarity and quantum yields of singlet oxygen and fluorescence.

The greater goal of my research is to help identify characteristics of hydroporphyrins that may contribute to the development of PDT probes that are more selective and sensitive in cellular environments. While measuring and graphing data may not seem like much in terms of demanding lab work, it can be very time consuming and requires meticulous tracking of volumes and careful protection of solutions from ambient light. I'm happy that my hard work paid off, though; I am an author in a paper being submitted for publishing right now, and I'm waiting on the acceptance of an abstract I submitted in order to present in the ACS National Meeting this fall!

Describe the process of obtaining your internship, research, or co-op opportunity.

I was actually a senior in Centennial High School when I obtained this research position. I was a student in Biotechnology Academy, a two-year G/T biotechnology career preparation program offered by the HCPSS Applications and Research Laboratory. Through this program I learned fundamental biological/chemical research and laboratory skills that allowed me, in my second year, the opportunity to find a position in a real scientific research lab. Among the options I had, I was most interested in Dr. Ptaszek's lab because I had no real knowledge of organic chemistry research and it sounded interesting. Dr. Ptaszek accepted me in October 2014 and I have been a lab member ever since.

What have you enjoyed the most about your position or organization/company?

I enjoy the challenge! As a freshman with almost no background in organic chemistry or quantum mechanics, it has been an intense journey to bring myself up to speed on the fundamentals of these topics, along with familiarity of the past few decades of hydroporphyrin research. It is immensely satisfying when I am able to explain to myself in minute detail why certain observations are the way they are, and it is even more rewarding when I am able to discuss my research with professionals in organic chemistry fields. There is still a great deal of information that I have not yet been able to understand, so it is also a humbling experience to admit that this challenge will be one I cannot master anytime soon.

How do you believe you have made an impact through your work?

I hope that the greatest contribution of my work will be to the improvement of photodynamic therapy and related "light-utilizing" cancer therapies, which have the potential to be minimally-invasive and non-toxic treatment options. One way to improve PDT probe specificity is to develop ones with behaviors sensitive to certain intra-cellular regions; this is what I am trying to address with solvent polarity dependence, as a cell has regions of differing polarities. The international interest in the advancement of PDT probes has grown steadily over time, so research in this field really has an impact on prospects for tightly regulated therapies in the future.

What advice would you give to another student who is seeking an internship or similar experience?

To a student (shout out to all the freshmen doing research!) interested in doing research but with little background knowledge of the topic the research lab focuses on: it can be done! There is a lot of implicit personal accountability for being able to understand what the research is, how and why it is being done, but it is far less of a burden if you are genuinely interested in the research instead of just adding a "professional experience" to a resume. Have confidence in yourself and know that you can only go so far as you are willing to try.