Vida Rahmatnejad, 'M.S. Chemical and Biochemical EngineeringPh.D. '23 Chemical and Biochemical Engineering with Michael Tolosa, Xudong Ge & Govind Rao have a new paper, published in the Biotechnology Letters. The title of the paper is: "Completely noninvasive multi-analyte monitoring system for cell culture processes".
Publication is available here
https://link.springer.com/article/10.1007/s10529-024-03521-zAbstract:
Although online monitoring of dissolved O2, pH, and dissolved CO2 is critical in bioprocesses, nearly all existing technologies require some level of direct contact with the cell culture environment, posing risks of contamination. This study addresses the need for an accurate, and completely noninvasive technique for simultaneous measurement of these analytes. A “non-contact” technique for simultaneous monitoring of dissolved O2, pH, and dissolved CO2 was developed. Instead of direct contact with the culture media, the measurements were made through permeable membranes via either a sampling port in the culture vessel wall or a flow cell. The efficacy of the “non-contact” technique was validated in Escherichia coli (E.coli), Chinese hamster ovary (CHO) culture processes, and dynamic environments created by sparging gases in cell culture medium. The measurements obtained through the developed techniques were comparable to those obtained through control methods. The noninvasive monitoring system can offer accurate, and contamination-minimized monitoring of critical process parameters including dissolved O2, pH, and dissolved CO2. These advancements will enhance the control and optimization of cell culture processes, promising improved cell culture performance.
A PDF of the report with images, is available using the "Download Document" button at the bottom of the story on the myUMBC post.
Tithi Prajapati, Krisha Pandya, Kalina Kostova
Tithi Prajapati is a rising undergraduate sophomore pursuing a Bioinformatics and Computational Biology degree at the University of Maryland, Baltimore County. She is a Meyerhoff Scholar at UMBC. Her program supports the mission of increasing the number of Ph.D. for minorities in the STEM field. Being part of such a program, and her dedication to obtaining a Ph.D., she has decided to get research experience to learn the skills and techniques which will be critical in her educational journey. She started working in the Center of Advanced Sensor Technology (CAST) because she found that CAST had various projects where she could learn various skills, both biological and technological, which are fit for her major. She enjoys working with CAST and being part of such a diverse and welcoming group. Being here only for a short time so far, she has learned multiple things -- working on the nitrate absorbance data, CO2 sensor, and creating microfluidic chips -- and she can't wait to expand her knowledge.
Krisha Pandya is a rising high school senior at Franklin High School, Baltimore County. An avid member of the Science Olympiad chapter at her school, she has found a passion for STEM over the three years of her participation there. She was introduced to CAST this summer and has assisted in several projects, under the guidance of amazing mentors. Krisha has worked with the time to conduct trial experiments for CO2 sensors and nitrate testing in tap water and DI samples. She now looks forward to planning her next academic year, applying to colleges.
Kalina Kostova is a rising freshman in high school, attending Centennial High School in Howard County, Maryland. While she might not have much experience in the STEM field, she has always been curious about it and is eager to expand her knowledge. During her internship at CAST, she helped collect nitrate absorbance data. Kalina is very grateful for everything she was taught by her mentors and for their responsiveness to any issues she faced during this experience. She is interested in pursuing a career in STEM in the future and looks forward to working with others in similar fields.
STEM is an acronym grouping together fundamental interdisciplinary principles: Science, Technology, Engineering, and Mathematics. It is a commonplace term pertinent to the conversation of the various technical undergraduate degrees and career possibilities today.
To understand the situation in STEM, we interviewed a few women in STEM. In the interview, they all gave similar responses to the question, "how do you maintain a good balance between your personal and professional life?"
Revati Kadolkar, a graduate student at the University of Maryland-Baltimore County said, "it is a challenge."
Like the other interviewees, she describes that the gender gap is very much prominent and that the household duties do eventually take a toll. Kadolkar positively affirms the importance of consistency though -- women must enable themselves to be consistent through their work to create their impact.
STEM fields have been primarily male-dominated, despite some developments in the 20th and 21st centuries[1]. From an MIT study, in 2023, the percentage of women in the STEM workforce comes to a marginal 28% around the world. When comparing statistics of other big nations specifically, we see that the United States has 24%, European Union has 17%, Japan has 16%, and India has 14% women in the STEM workforce [3]. Hence, not just in the US, this is a worldwide problem. Women are largely seen working lower-level administration positions at lower pay rates than their male counterparts.
Statistics show women in healthcare comprise 80% of total employment, but a closer look at the statistics evidences only 21% of this population holds upper executive positions even in healthcare [6]. Women are much less likely to have stature enabling them as authoritative figures responsible for decision-making and upper management. Their skill sets are undermined evident by the continual absences of more women in the engineering and computational science areas, and surgery.
In an interview with Dr. Sadhika Jamisetti, an Assistant Professor in the Department of Family and Community Medicine at UT Southwestern Medical Center, she points out, "In terms of female to male ratio, we actually have more females, because I'm in family medicine, but if you get out of family medicine and you go to different specialties, like surgical, especially surgical, might not see as many women...". She still believes there is hope, and aspires that one day there will be more women in STEM. Excelling in multiple specialties, not confined to just one.
Dr. Prerna Joshi, working more at the technical forefront, also feels similarly. A senior scientistelectrochemistry at Xerion Advanced Battery Corp in Ohio, she says, "In the field of electrochemistry I have not seen more women, they either go in biology as in pursuing medicine or in chemistry as in polymer related things but they don't usually go towards the physics kind of field in science." But she too hopes that this problem will be fixed soon.
Women are more involved in the social standpoint of the world. They are more likely to read blogs, make up a huge audience of social media content readers, and generally they tend to stay more engaged in world affairs than men [4]. Having such strong involvement adds value to their perspective and their activeness and dedication proves to be of significant value towards the society. A strong attribute of females that STEM cannot put away. Not just females, diversity in STEM is critical as a whole.
When seeking a solution to challenges and problems faced, the lack of diversity in STEM acts as a barrier. Increasing diversity in the STEM field will bring new ideas and creativity, which will only help foster more development within STEM. This development is only possible when multiple perspectives are taken into account. STEM should be more inclusive of minority groups including not just women, but marginalized racial groups and children.
With the many opportunities and career choices today, lies the future of the new generation. This new generation needs to be informed on how they can contribute to the advancement and progression of their lives by being part of STEM. Diversifying STEM is diversifying an ideology that promotes stronger collaboration and unity.
Dr. Sai Kiran Mani, how she found her way
"It's amazing how different minds think differently. [It makes for] a dynamic environment."
As postdoctoral fellow at UMBC, Dr. Sai Kiran Mani focuses her research on the development of low-cost and effective sensors for water samples, specifically nitrate sensor. She is a strong believer that passion drives an individual and firmly stands for collaboration in the workspace.
According to her, having mentors and experts who are willing to give their guidance helps alleviate the pressure of benchwork in STEM.
Dr. Preety Ahuja, on why she chose STEM
Dr. Preety Ahuja is an Assistant Research Scientist at UMBC with a Ph.D. in Electrochemistry from the University of Delhi, India. A reputed member of the Center for Advanced Sensor Technology (CAST) here at UMBC and as a mother, Dr. Ahuja has shifted her research focus to the development of non-invasive room-temperature CO2 monitors for neonates.
Dr. Ahuja decided she wanted to make safer sensors that would not be irritable to newborns with sensitive skin. Current CO2 sensors for newborn care are within the temperature ranges of 44-45 degrees Centigrade; these temperatures are extreme for younger children, especially neonates [7].
In her statement, she mentions she wanted to do this for her daughter. She has first-hand witness of the technologies used for neonate care and opines that huge improvements are needed within healthcare especially on technologies for postnatal and neonatal care. She aspires to initiate the change through CAST.
At CAST, she has the freedom to be able to begin her project. The reason Dr. Ahuja was able to do this is because she had the opportunity to do so -- something not many women have access to.
Dr. Pegah Rezaei, on her journey to the US
Dr. Pegah Rezaei has been part of the CAST team at UMBC. She described her experience in Medical School in Turkey, where medical students from neighboring countries would come to Turkey to study. She realized the differences in medical advancement even between borders, not everyone has the same access to medicine.
To explore this, she got interested in research, and came to the US to start her post doctorate after medical school. As part of her research, she has been working on developing the CO2 monitoring device relevant to the escalating opioid crisis.
These three women with three different backgrounds yet they come together to work in a lab in another country. Together they continue to show their knowledge and motivation towards research by being strong leaders in their respective fields, working towards the creation of low-cost and highly effective sensors such as nitrate and CO2 sensors.
There are fewer women relative to men in STEM. Even fewer minority-based women. There is a need for regulation in underdeveloped policies and agendas which facilitates a culture where women are discredited and remain segregated in the opportunities they are given to succeed. To say the least, America has yet to cross the 50% benchmark but there is still change to come about, slowly but surely.
We would like to thank Dr. Venkatesh Srinivasan, the Assistant Professor at CAST, for giving us the opportunity to write this paper and for setting up interviews. We would like to extend our appreciation to the interviewees, Dr. Preeti, Dr. Sai Kiran Mani, Dr. Sadhika, Dr. Joshi, Dr. Pegah, and Revati Kadolkar. They have all been a great source of inspiration for us.
We lastly extend our gratitude to Dr. Govind Rao, the Director and Chairman of CAST, who has welcomed us to be a part of the CAST team. Thank you.
1. Environmental Protection Agency. (n.d.). EPA. https://www.epa.gov/perspectives/whyrepresentation-matters-girls-and-women-stem
2. Filed in Employment Trends Data and Technology Career Information By: Emily Krutsch, V. R. - N. 4. (n.d.). Stem day: Explore growing careers. DOL Blog. https://blog.dol.gov/2022/11/04/stem-day-explore-growing-careers
3. The gender gap in Stem. MIT Professional Education. (2023, July 12). https://professionalprograms.mit.edu/blog/leadership/the-gender-gap-in-stem
4. Posts. (2022, March 2). The importance of women in STEM: Why diversity matters. Her Culture. https://www.herculture.org/blog/2022/3/2/the-importance-of-women-in-stem-whydiversity-matters
5. Special topics annual report: Women in the stem. US EEOC. (n.d.). https://www.eeoc.gov/special-topics-annual-report-women-stem
6. The stem gap: Women and girls in Science, Technology, Engineering and Mathematics. AAUW. (2022, March 3). https://www.aauw.org/resources/research/the-stem-gap/
7. Use of transcutaneous CO2 monitoring in NICU (May 2019). (n.d.). https://www.ashfordstpeters.net
Photo credit: Tithi Prajapati. Krisha Pandya, Kalina Kostova and Tithi Prajpati (From left to right)
Erick Gutierrez was selected as the recipient of the Young Professionals Award in Upstream and Downstream Processing for his poster “Microscale Chromatography toolkits for rapid screening and purification of therapeutic proteins” presented at the 2019 Orlando Meeting.
This award recognizes excellence by a non-student member of the BIOT 3M Separation and Purification Sciences Division and ACS-BIOT (American Chemical Society’s Division of Biochemical Technology) for Young Professionals in Upstream & Downstream Processing. This award is given to professionals 35 years of age or younger who present outstanding research in upstream and downstream processing at the poster session as part of the Division of Biochemical Technology (BIOT) programming at the ACS annual meeting. The 3M Company is the sponsor for this award.
The 2019 BIOT Award for Young Professionals in Upstream & Downstream Processing will be awarded as part of 2020 Spring National Meeting, in Philadelphia, PA from March 22 - 26.
Erick graduated from UMBC with a Bachelor's degree in Chemical Engineering (’18). He worked at Center for Advanced Sensor Technology (CAST) located in UMBC, as a Systems Design Engineer focused on the product development of microfluidic devices used for downstream processing of therapeutic proteins.
He began his career in microfluidics by working as the team leader of an undergraduate team in Dr. Govind Rao's sensors class, tasked with developing a microfluidic debubbler for his Bio-MOD project funded by DARPA. He was then offered an internship position in the systems design team of the Bio-MOD project under the mentorship of Dr. Abhay Andar, where he was tasked with optimizing and making the bioprocesses used for the G-CSF purification runs, as well as developing, manufacturing, and validating microfluidic devices for downstream processing.
Upon graduation, he was offered a full-time position as a Research Associate in the Bio-MOD project, joining for the second phase of the project in which we had to optimize our process for animal studies. We developed several microfluidic devices, such as mixers, holders, novel and fully customizable chromatography micro-columns (µcols), and multi-functional integrated chips using thermo-plastic solvent bonding methods and adhesives. Our work for "Low‐cost customizable microscale toolkit for rapid screening and purification of therapeutic proteins," was recently published. (https://doi.org/10.1002/bit.26876) We further expanded our microfluidics research by using wood instead of plastics as our primary material, which led to the publication of “Wood Microfluidics,” published in Analytical Chemistry in 2019.
]]>